rotem® delta analyser (Pentapharm)
Structured Review

Rotem® Delta Analyser, supplied by Pentapharm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rotem%C2%AE+delta+analyser/rotem++delta+analyser/pmc03416788-155-5-8
Average 90 stars, based on 1 article reviews
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1) Product Images from "Bivalirudin in Combination with Heparin to Control Mesenchymal Cell Procoagulant Activity"
Article Title: Bivalirudin in Combination with Heparin to Control Mesenchymal Cell Procoagulant Activity
Journal: PLoS ONE
doi: 10.1371/journal.pone.0042819
Figure Legend Snippet: -hALPCs PCA in ROTEM (Blood). Clotting time (CT) assayed by ROTEM after recalcification, with added tissue factor (ExTem 20 µL) of citrated whole blood (300 µl) in presence or not of cells suspended in human albumin 5%. No coagulation was induced in the absence of recalcification. Hepatocytes (white), human adult liver progenitor cells (hALPCs) (black), control (albumin) (grey). * as compared to hALPCs f as compared to control hALPCs vs. hepatocytes vs. control. ***p<0.001 (Kruskal-Wallis test). -hALPCs PCA in ROTEM (Plasma). Clotting time (CT) assayed by ROTEM after recalcification, with added tissue factor (ExTem 20 µL) of plasma (300 µl) obtained from blood incubated in presence or not of cells suspended in human albumin 5%. Hepatocytes (white), human adult liver progenitor cells (hALPCs) (black), control (albumin) (grey). * as compared to hALPCs f as compared to control hALPCs vs. hepatocytes vs. control. ***p<0.001 (Kruskal-Wallis test). -hALPCS PCA in ROTEM (no TF addition). Clotting time (CT) essayed by ROTEM after recalcification, without added Tissue Factor (ExTem 20 µL), of citrated whole blood (300 µl) in presence or not of cells suspended in human albumin 5%. No coagulation is induced if absence of recalcification. Hepatocytes (white), hALPCs (black).
Techniques Used: Coagulation, Incubation
Figure Legend Snippet: Clotting time (CT) assayed by ROTEM after recalcification, with added tissue factor (ExTem 20 µL) of citrated whole blood (300 µl) with human adult liver progenitor cells (hALPCs), hepatocytes, skin fibroblasts, bone marrow mesenchymal stem cells (BMMSC), bone marrow haematopoietic stem cells (BMHSC), or liver myofibroblasts suspended in human albumin 5% f as compared to control.
Techniques Used: Coagulation
Figure Legend Snippet: Clotting time (CT) assayed by ROTEM after recalcification, with added tissue factor (ExTem 20 µL) of plasma (300 µl) deficient in coagulation factors VII, V, X, and II (7d Pl, 5d Pl, 10d Pl, and 2d Pl, respectively) in presence of cells suspended in human albumin 5%. Human adult liver progenitor cells (hALPCs) (black), control (albumin) (grey). * as compared to normal plasma f as compared to control.
Techniques Used: Coagulation
Figure Legend Snippet: Clotting time (CT) assayed by ROTEM after recalcification, with added tissue factor (ExTem 20 µL) of citrated whole blood (300 µl) in presence or not of human adult liver progenitor cells (hALPCs) suspended in human albumin 5% with heparin (Hepar). In contrast, enoxaparin (Eno) or Fondaparinux (Fond) was extemporaneously added to blood in contact with cells suspended in albumin. hALPCs (black), Control (albumin) (grey). * as compared to hALPCs f as compared to control Clotting time (CT) assayed by ROTEM after recalcification, with added tissue factor (ExTem 20 µL) of citrated whole blood (300 µl) in presence or not of human adult liver progenitor cells (hALPCs) suspended in human albumin 5%. Bivalirudin (Biva) or Hirudin (Hir) was extemporaneously added to blood. hALPCs (black), Control (albumin) (grey). * as compared to hALPCs f as compared to control Clotting time (CT) assayed by ROTEM after recalcification, with added tissue factor (ExTem 20 µL) of citrated whole blood (300 µl) in presence or not of hepatocytes suspended in human albumin 5% with heparin (Hepar). In contrast, enoxaparin (Eno) or Fondaparinux (Fond) was extemporaneously added to blood in contact with cells suspended in albumin. Hepatocytes (white), Control (albumin) (grey). * as compared to hepatocytes f as compared to control Clotting time (CT) assayed by ROTEM after recalcification, with added tissue factor (ExTem 20 µL) of citrated whole blood (300 µl) with or without hepatocytes suspended in human albumin 5%. Bivalirudin (Biva) or Hirudin (Hir) was extemporaneously added to blood. Hepatocytes (white), Control (albumin) (grey). * as compared to hepatocytes f as compared to control.
Techniques Used: Coagulation
Figure Legend Snippet: Clotting time (CT) assayed by ROTEM after recalcification, with added tissue factor (ExTem 20 µL) of citrated whole blood (300 µl) in presence or not of human adult liver progenitor cells (hALPCs) suspended in human albumin 5% with heparin (Hepar), enoxaparin (Eno), or fondaparinux (Fond) extemporaneously added to blood. Combination of anticoagulant drugs was obtained when bivalirudin (Biva) was extemporaneously added to blood. hALPCs (black), Control (albumin) (grey). * as compared to hALPCs f as compared to control $ as compared to bivalirudin Clotting time (CT) assayed by ROTEM after recalcification, with added tissue factor (ExTem 20 µL) of citrated whole blood (300 µl) in presence or not of hepatocytes suspended in human albumin 5% with heparin (Hepar), enoxaparin (Eno), or fondaparinux (Fond) extemporaneously added to blood. Combination of anticoagulant drugs was obtained when bivalirudin (Biva) was extemporaneously added to blood. Hepatocytes (white), Control (albumin) (grey). * as compared to hepatocytes f as compared to control $ as compared to bivalirudin.
Techniques Used: Coagulation